A novel Coronavirus, currently termed 2019-nCoV or COVID-19, was announced as the etiological agent of cases of ongoing Pneumonia outbreak in Wuhan, China. The Assay Genie Coronavirus COVID-19 (Strain 2019-nCoV) One-Step RT-qPCR Detection Kit is used for the in vitro detection of 2019-nCoV or COVID-19 in respiratory specimens (sputum; nasopharyngeal, oropharyngeal aspirates, washes or swabs; tracheal aspirates) using real time RT qPCR.
The Assay Genie Coronavirus COVID-19 (Strain 2019-nCoV) One-Step RT-qPCR Detection Kit allows efficient cDNA synthesis and Real-Time PCR in a single tube. The kit includes a qPCR MasterMix supplied in a 2x concentration to perform real-time PCR. qPCR MasterMix (2x) contains all the reagent (except PCR primers, probe and template) needed for running PCR reactions. In addition, a separate RT Mix that comprises a balanced mixture of both RTase (Reverse Transcriptase) and RNase Inhibitor is also provided.
Additionally, the kit contains one set of Primers and Fluorescent Probes to differentiate between 2019-nCoV and SARS-CoV (or bat SARS related CoV). Coronavirus 2019-nCoV RNA targets are amplified and detected in the FAM channel while Coronavirus SARS-CoV (or bat SARS related CoV) RNA targets are amplified and detected in the HEX, VIC or JOE channel (depending on the equipment used).
Also, it includes a Positive Control (PTC) and Negative Control (NTC). The positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a negative control reaction should be included every time the kit is used.
- Relevant costs-savings: in comparison with other suppliers.
- Easy and fast detection by qPCR.
- Inclusivity: SARS like coronavirus and specific detection of 2019-nCoV.
- Dual Color Multiplex Assay Format.
- Products in stock: Ready-to-ship immediately.
- Complete solution: includes Positive and Negative controls.
- Versatile: compatible with most used real-time PCR instruments.